Cell adhesion phenotyping by quantitative polymerase chain reaction in melanoma: Clarifying misconceptions

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Endothelial Cell Isolation and Quantitative Polymerase Chain Reaction

Cerebral cavernous malformations (CCMs) are common vascular malformations that arise primarily in the central nervous system. CCMs are typically diagnosed in middle age and constitute an important cause of stroke and neurological deficit in younger individuals. One third of CCMs are familiar, and positional cloning studies have identified loss of function mutations in 3 genes, KRIT1, CCM2 and P...

Plasmid Copy Number Determination by Quantitative Polymerase Chain Reaction

Recombinant therapeutic proteins are biopharmaceutical products that develop rapidly for years. Recombinant protein production in certain hosts requires vector expression harboring the gene encoding the corresponding protein. Escherichia coli is the prokaryote organism mostly used in recombinant protein production, commonly using a plasmid as the expression vector. Recombinant protein productio...

Diagnosis of tubercular uveitis by quantitative polymerase chain reaction

PURPOSE To report the use of real-time or quantitative polymerase chain reaction (qPCR) in confirming the diagnosis of tubercular uveitis. METHODS A qPCR assay using primers targeting the MPB64 gene of Mycobacterium tuberculosis (MTB) was developed. A standard curve for quantification of mycobacterial load was generated. Vitreous fluid samples from patients presumed to have tubercular uveitis...

SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION

Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...